Fig. 6: SNPH regulates lamellipodia formation and metastasis of CRC cells through ROS-modulated activity of the cdc42-PAK1-Cofilin pathway. | Cell Death & Disease

Fig. 6: SNPH regulates lamellipodia formation and metastasis of CRC cells through ROS-modulated activity of the cdc42-PAK1-Cofilin pathway.

From: HIF-1α suppresses SNPH expression to facilitate liver metastasis of colorectal cancer through regulating mitochondrial dynamics and filopodia formation

Fig. 6: SNPH regulates lamellipodia formation and metastasis of CRC cells through ROS-modulated activity of the cdc42-PAK1-Cofilin pathway.The alt text for this image may have been generated using AI.

A The intracellular ROS levels in CRC cells treated with H2O2 or Mito-TEMPO were assessed using flow cytometry. B Western blot analyses were performed to determine the protein levels of AKT, p-AKT, ERK, and p-ERK in CRC cells treated as indicated. C Western blot analyses for protein levels of AKT, p-AKT, cdc42, cdc42GTP, PAK1, p-PAK1 (Thr423), Cofilin, p-Cofilin (Ser3) in SNPH overexpressed Caco2 treated with or without H2O2. D Western blot analyses for protein levels of AKT, p-AKT, cdc42, cdc42GTP, PAK1, p-PAK1 (Thr423), Cofilin, p-Cofilin (Ser3) in SNPH knockdown RKO treated with or without Mito-TEMPO. E, F Phalloidin staining in Caco2 and RKO as indicated. Triangles indicate lamellipodia formation. Scale bars, 5 µM. *P < 0.05; **P < 0.01; ***P < 0.001.

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