Fig. 3: Analysis of BCLAF1 expression and epigenetic changes in DNMT3A knockout U937 clones.
From: Dynamic epigenetic regulation of BCLAF1 splicing in acute myeloid leukemia
A GEPIA analysis on DNMT3A expression in AML patient samples. B Real-time PCR and Western blot analyses of DNMT3A expression in U937 sh-DNMT3A clones after doxycycline induction (2 µg/mL) at 6, 24, and 48 h. C Cell proliferation assay by cell counting in U937 sh-DNMT3A clones after doxycycline induction (2 µg/mL) at 6, 24, and 48 h. D PCR analysis of BCLAF1 expression in U937 sh-DNMT3A clones after doxycycline induction (2 µg/mL) at 6, 24, and 48 h. E ChIP analysis performed in U937 sh-DNMT3A clones after 48 h of doxycycline induction (2 µg/mL) on BCLAF1 promoter and exon 5 regions against HDAC1, DNMT3A, DNMT3B, MRG15, and RNA Polymerase II (Ser5P), and histone modifications H3K36me3, H3K4me3, H3K27me3, and H3K9/14ac. Values are presented as the mean ± SEM. Significance was defined as a p-value < 0.05, indicated by asterisks in the figures (* = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001).
