Fig. 6: PRMT7-mediated R76 methylation of GLUD1 promotes gastric cancer proliferation and migration.
From: AKT1 phosphorylates PRMT7 to promote GLUD1 methylation and gastric cancer progression
A–D GLUD1 knockdown reduces gastric cancer cell proliferation and migration, which is rescued by re-expression of wild-type GLUD1 but not R76K mutant. Western blot analysis of Flag-GLUD1 (WT/R76K) re-expression in AGS cells following GLUD1 knockdown (A); Proliferation rates by CCK-8 assay (B) and EdU-positive cell quantification (C); Wound healing assay with migration rates quantified (D). E–G AdOx suppresses cell proliferation and migration dose-dependently. AGS cells were treated with or without 20 or 50 μM AdOx for 24 h followed by CCK8 assay (E), EdU assay (F) and wound healing assay (G). H–J SGC3027 suppresses proliferation and migration. AGS cells or GLUD1 knockdown cells with re-expression of GLUD1 WT or R76K mutant were treated with or without 10 μM SGC3027 for 24 h followed by CCK8 assay (H), EdU assay (I) and wound healing assay (J). Scale bar: 50 μm. Data are presented as mean ± SD. (n = 3, unpaired two-tailed t-test, **p < 0.01, ***p < 0.001, ****p < 0.0001).
