Fig. 3: ACAD9 decreases ROS production by promoting the assembling of mitochondrial complex I and SCs, thereby inhibiting osteoclast differentiation. | Cell Death & Disease

Fig. 3: ACAD9 decreases ROS production by promoting the assembling of mitochondrial complex I and SCs, thereby inhibiting osteoclast differentiation.

From: Moonlighting cytosolic function of ACAD9: suppression of TRAF6-mediated osteoclastogenesis and protection against osteoporosis

Fig. 3: ACAD9 decreases ROS production by promoting the assembling of mitochondrial complex I and SCs, thereby inhibiting osteoclast differentiation.The alternative text for this image may have been generated using AI.

ROS levels were detected using DCFH2-DA in the cells. A ROS levels from cells that stably knockdown ACAD9 after induced 4 days. B ROS levels from cells that overexpressed Acad9 after induced 4 days. C Mitochondrial complex I–V were detected by western blotting from RAW264.7 cells. Mitochondrial complex subunit I was detected from cells that (D) stably knockdown Acad9 or (E) overexpressed Acad9. F G-MAD revealed the potential role of ACAD9 in bone and bone marrow tissues. G, L, N Immunoblots for SCs by BN-PAGE and immunoblot with NDUFA9 (subunit of Complex I) and (H), (M), (O) concentrations of ATP were detected by assay kit in the indicated RAW264.7 cells. AlphaFold3 predicted the interaction of complex subunits I/II/III I in the absence of ACAD9, or (J) in the presence of ACAD9, as shown. K Co-IP analysis on the interaction between ACAD9 and complex subunits of I, II, III, and IV. Data are shown as Mean ± SEM, ∗p < 0.05, ∗∗ p < 0.01 vs. Ctrl group; # p < 0.05, ##p < 0.01 vs. Ctrl+RANKL group. For in vitro experiments, data represent at least three independent experiments.

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