Fig. 1: Generation and characterization of G-heporgs from hESCs. | Cell Death & Disease

Fig. 1: Generation and characterization of G-heporgs from hESCs.

From: Generation of proliferative hESC-derived grape-clustered hepatocyte organoids with multipolar architecture as regenerative counterpart via synergy of YAP and IGF2 pathways

Fig. 1: Generation and characterization of G-heporgs from hESCs.The alt text for this image may have been generated using AI.

A Schematic illustration of the generation of G-heporgs and S-heporgs from hESC-derived HB-orgs. B Representative morphologies of HB-orgs, G-heporgs and S-heporgs. Scale bar = 100 μm. C Immunostaining of ALB and Ecad in indicated groups. Nuclei were stained with DAPI. Yellow arrows indicated binucleated hepatocytes. Scale bar = 50 μm. D The quantification of ALB expression intensity. n = 4 biologically independent experiments. E The analysis of the secretion of ALB in indicated groups. n = 4 biologically independent experiments. F Immunostaining of Ki67, Ecad, Factin, and ZO1 in indicated groups. Nuclei were stained with DAPI. Scale bar = 50 μm. G Representative immunostaining and 3D reconstruction images for ZO1 in indicated groups. Nuclei were stained with DAPI. Scale bar = 50 μm. Results were presented as mean ± SD. Statistical significance was determined using one‑way ANOVA followed by Tukey post‑test. ***p < 0.001.

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