Fig. 2: Comparison between G-heporgs and S-heporgs. | Cell Death & Disease

Fig. 2: Comparison between G-heporgs and S-heporgs.

From: Generation of proliferative hESC-derived grape-clustered hepatocyte organoids with multipolar architecture as regenerative counterpart via synergy of YAP and IGF2 pathways

Fig. 2: Comparison between G-heporgs and S-heporgs.

A Volcano plot analysis of G-heporgs and S-heporgs. B Analysis of KEGG pathway of G-heporgs versus S-heporgs. C Heatmap of G-heporgs and S-heporgs for genes related to proliferation, mature, YAP, and necroptosis and senescence. D Immunostaining of Factin and lipid droplet (BODIPY staining) in indicated groups. Nuclei were stained with DAPI. Scale bar = 50 μm. E Analysis of cell senescence by SA-β-Gal staining. Scale bar = 100 μm. F Analysis of cell apoptosis by TUNEL staining. Nuclei were stained with DAPI. Scale bar = 100 μm. G GSEA analysis. H Immunostaining of Factin and YAP in indicated groups. Nuclei were stained with DAPI. Scale bar = 50 μm. Yellow arrows indicate YAP with nuclear localization, red arrows indicated YAP without nuclear localization. I Quantification of YAP nuclear localization. n = 4 biologically independent experiments. J Heatmap of G-heporgs and S-heporgs for genes related to cytokines and chemokines. Results were presented as mean ± SD. Statistical significance was determined using unpaired two-tailed Student’s t test. ***p < 0.001.

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