Fig. 6: Characterization of the multi-polarization structure of expandable G-heporgs. | Cell Death & Disease

Fig. 6: Characterization of the multi-polarization structure of expandable G-heporgs.

From: Generation of proliferative hESC-derived grape-clustered hepatocyte organoids with multipolar architecture as regenerative counterpart via synergy of YAP and IGF2 pathways

Fig. 6: Characterization of the multi-polarization structure of expandable G-heporgs.

A Immunostaining of Factin, ZO1, MRP2, and MDR1. Nuclei were stained with DAPI. Scale bar = 50 μm. B, C 3D imaging of MDR1 and Factin staining. D Representative images of transmission electron micrograph (TEM). Scale bar = 2 μm (left) and 500 nm (middle and right). Red arrows indicated microvilli, blue arrows indicated tight junction and yellow arrows indicated desmosomes. E Representative images of CDFDA staining. Scale bar = 100 μm. F Representative images of Rho123 staining with or without Ver (Verapamil) treatment. Scale bar = 50 μm.

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