Fig. 2: Landscape of m6A and its regulators in DIPGXIII and its isogenic lines. | Cell Death & Disease

Fig. 2: Landscape of m6A and its regulators in DIPGXIII and its isogenic lines.

From: RNA N6-methyladenosine (m6A) regulates cell cycle progression in diffuse midline glioma (DMG) and confers sensitivity to FTO inhibition

Fig. 2: Landscape of m6A and its regulators in DIPGXIII and its isogenic lines.

A Scatterplots of DRACH m6A levels in SU-DIPGXIII (K27M) versus its isogenic K27M-knockout line across the 5′UTR, CDS (internal), and 3′UTR. White numbers: number sites with >10% difference (above/below red solid line). Red numbers: sites with >%20 difference (above/below red dotted line). Sites are filtered for >10 coverage and >10% average m6A in one condition. B Expression levels (TPM) of METTL3, ALKBH5 and FTO in SU-DIPGXIII and its isogenic lines. Error bars represent standard error. C Relative expression levels of METTL3, ALKBH5, and FTO in SU-DIPGXII and its isogenic line after 3 days of culturing in stem vs differentiation media. Error bars represent standard error. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001. D Average transcript levels (log2(CPM + 1) of m6A readers in different cancers and non-malignant cells [48]. Adult high-grade gliomas (Adult HGG; n = 16), typical teratoid rhabdoid tumors (ATRT; n = 19), H3G34-mutant diffuse hemispheric gliomas (H3G34-DHG; n = 6), H3K27-altered diffuse midline gliomas (H3K27-DMG; n = 51), H3WT - high grade gliomas (H3WT-HGG; n = 19), Medulloblastoma (n = 7), malignant rhabdoid tumors (n = 7), non-malignant (n = 39). The boxplots show the median, first and third quartiles with the whiskers extended to the last point within 1.5X of the interquartile range. Red dotted line indicates cohort median. P-value for significant difference from median shown: *<0.05, **<0.01, ***<0.001, ****<0.0001.

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