Fig. 6: Chromatin accessibility changes in response to matrix stiffness. | Cell Death & Disease

Fig. 6: Chromatin accessibility changes in response to matrix stiffness.

From: Stiff matrix-induced KRTAP2-3 expression suppresses ciliogenesis via actin tension-driven chromatin remodeling

Fig. 6: Chromatin accessibility changes in response to matrix stiffness.The alternative text for this image may have been generated using AI.

A ATAC-seq workflow schematic. B Differential accessible region upstream of the KRTAP2-3 gene locus between 0.1 and 40 kPa matrix. Scale bars, 500 bp. C The locations of primers for ATAC-qPCR are shown as indicated. D RPE-1 cells grown on matrix of the indicated stiffness were treated with DMSO, cytochalasin B (Cyto B), or cytochalasin D (Cyto D) for 6 h were analyzed by ATAC-qPCR. Data show allele-specific accessibility at the KRTAP2-3 locus under different treatments. Normalized to 9490. 9490 region is a constant gene locus (located at chr1: 241683128-241683278 according to hg19) in 0.1/40 kPa matrix stiffness according to ATAC-seq data, which is designed by ATAC Primer Tool (APT). Quantitative data are presented as mean ± SD, two-tailed Student’s t test. Experiments were performed with at least two biological replicates.

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