Fig. 1: BIRC5hiCD4+ cells represent active insulin-sensitive clusters in RA tissues.
From: Insulin enables acquisition of the IL7R+ memory phenotype in PD1+ T cells in RA tissues
A Glucose metabolism. Studied metabolic pathways are in bold. B Box plots of standardized expression sum (SES), by RNA-seq, of genes within metabolic pathways in CD4+ cells of RA (n = 11), healthy controls (HC, n = 57), and of CD4+ cells split by mean expression of BIRC5 gene in high (B5hi) and low (B5lo) (n = 24). Boxes indicate IQR and whiskers indicate the minimum and maximum values. GO-terms used for pathway annotation: Glycolysis (GO:0006096); oxidative phosphorylation (OxPhos, GO:0006119 and GO:0022900); pentose phosphate pathway (PPP, GO:0006098); tricarboxylic acid cycle (TCA, GO:0006099). P-values are calculated by Wilcoxon unpaired test. C Heatmap of Spearman’s correlation between HAT, HDAC and metabolic pathways as above. GO-terms used for annotation HAT (GO:0000123) and HDAC (GO:0000118). Asterisks indicate p-values. * < 0.05, ** < 0.01, *** <0.001. D Heatmap of Spearman’s correlation between HAT and HDAC and insulin signaling (IS). Asterisks indicate p-values * < 0.05, ** < 0.01, *** <0.001. E Scatter plot of Spearman’s correlation between plasma insulin levels and SES of insulin signaling. F Uniform manifold approximation and projection (UMAP) map of scaled expression intensity of BIRC5, IFNG, and TNF in T cells of RA synovial tissue (ST), by single cell transcriptome. BIRC5HiCD4+ clusters are indicated. G Heatmap of scaled expression intensity of metabolic pathway (annotated as above) in peripheral blood (PB), synovial fluid (SF), and ST of RA patients, by scRNA-seq. H Violin plot of insulin signaling by SES of INSR, IRS1, IRS2 and IGF1R genes in BIRC5HiCD4+ clusters. P-value was calculated by chi-square test. I Violin plot of metabolic pathways SES in cells with high and low IS in BIRC5HiCD4+ clusters. P-values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001 J. Box plot of BIRC5, IFNG, and TNF expression in Tph cells with high and low IS. Boxes indicate IQR and whiskers indicate the minimum and maximum values. P-values are calculated by Wilcoxon unpaired test. K Heatmap of expression for IL7R-signaling targets in Tph cells high and low IS. Genes regulated by survivin-H3K27ac are in bold. P-values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001.
