Fig. 3: Co-deposition of survivin-H3K27ac on chromatin controls transcription of the acetylation complex subunits.

A Analysis strategy of survivin and H3K27ac deposition, by ChIP-seq, in cis-regulatory elements (cis-RE) and connected genes in human CD4⁺ T cells. B Venn diagram of insulin-responsive (InsResp) genes connected to cis-RE with survivin-H3K27ac co-deposition. Genes are identified by DESeq2 analysis of CD4⁺ cells transcriptome after a) direct insulin stimulation (InsStim) and b) in regression to corresponding plasma insulin levels, by RNA-seq. C Scatter plot of pathway enrichment of insulin-responsive genes, by GSEA GO-terms. Circle size indicates the number of genes in the pathway, and color intensity indicates p-value. D Venn diagram of insulin-responsive (InsResp) genes connected to cis-RE with H3K27ac deposition. Genes are identified by DESeq2 analysis of CD4⁺ cells transcriptome after a) direct insulin stimulation (InsStim) and b) HDAC inhibition. E Scatter plot of protein subunits with survivin-H3K27ac in HAT (E1) and HDAC (E3) complexes, annotated by STRING database. Circle size represents number of cis-RE with survivin-H3K27ac connected to the gene. Circle color represents beta-coefficient of expression difference by DESeq2 analysis of CD4⁺ cell transcriptome in regression to corresponding plasma insulin levels. (E2 and E4) Heatmap of transcription difference, by log2FoldChange (FC), in CD4⁺ cells in regression to plasma insulin levels (InsReg), and after HDAC inhibitor treatment in vitro. F. Bar plot of percentage of H3K27ac and survivin-H3K27ac connected genes in each metabolic pathway. GO-terms used for pathway annotation as in Fig. 1. G Scatter plot of expression difference of survivin-H3K27ac connected genes in transcriptome of CD4⁺ cells in regression to plasma insulin levels and after HDACi treatment, by RNA-seq. Expression difference is calculated by DESeq2 analysis. Colour fill corresponds to metabolic pathway annotation of the gene.