Fig. 4: eIF2α expression is restored by bafilomycin and not by proteasome inhibitor
From: IRE1α deficiency promotes tumor cell death and eIF2α degradation through PERK dipendent autophagy
a Quantitative RT-PCR of eIF2α mRNA in U937 cells treated or not for 18 h with 4μ8 C (12.5 μM), TN (3 μM) or both drugs. The reported values are means ± S.D. (N = 3). Statistical analysis by Student’s t-test is shown. b, c Representative western blots out of three experiments are shown for t-eIF2α detected in the lysates of U937 cells treated or not with TN (3 μM), 4μ8 C (12.5 μM) or both drugs for 18 h and in the last 6 h treated or not with MG132 (50 μM) or with Bafilomycin (50 nM). β-actin is shown as loading control. The values under each band were obtained using the formula: (densitometry value of the band under investigation / densitometry value of the band of the corresponding β-actin) / (densitometry value of the band under investigation in the lysate of untreated cells / the densitometry value of the β-actin band in the lysate of untreated cells). d Quantitative analysis of total eIF2α detected in the western blots of three independent experiments was performed as above indicated. Results are expressed as mean ± S.D. and statistical analysis by Student’s t-test are shown
