Fig. 4: Late activated NF-κB is involved in the induction of TNFα expression and apoptosis by E2. | Cell Death Discovery

Fig. 4: Late activated NF-κB is involved in the induction of TNFα expression and apoptosis by E2.

From: Modulation of nuclear factor-kappa B activation by the endoplasmic reticulum stress sensor PERK to mediate estrogen-induced apoptosis in breast cancer cells

Fig. 4

a, b, MCF-7:5C cells were treated with vehicle control (0.1% EtOH) or E2 (1 nm) for the indicated periods. TNFα (a) and LTB (b) expression levels were quantitated by RT-PCR. *P < 0.05 and **P < 0.001 compared with control. c−e MCF-7:5C cells were treated with E2 (1 nm), JSH-23 (20 µm), or a combination of them for 72 h. TNFα (c), LTB (d), and Bim (e) expression levels were quantitated by RT-PCR. *P < 0.05 and **P < 0.001 compared with control. f MCF-7:5C cells were treated with E2 (1 nm), JSH-23 (20 µm), or a combination of them for 72 h. Cell lysates were harvested for western blotting using an anti-PARP antibody. g MCF-7:5C cells were transfected with a specific NF-κB siRNA for 72 h, using scrambled siRNA as control. Cell lysates were harvested for western blotting. h MCF-7:5C cells were treated with E2 (1 nm), JSH-23 (20 µm), or a combination of them for 72 h. Cells were harvested for Annexin V binding assay

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