Fig. 5: Functional characterization of the chimeric protein XIP-NLS. | Cell Death Discovery

Fig. 5: Functional characterization of the chimeric protein XIP-NLS.

From: Na+/Ca2+ exchanger 1 on nuclear envelope controls PTEN/Akt pathway via nucleoplasmic Ca2+ regulation during neuronal differentiation

Fig. 5

a Representative traces of Fura-2-detected [Ca2+]n after Na+-free perfusion in isolated nuclei prepared from NGF-differentiated PC12 cells previously transfected with mock or XIP-NLS. b Quantification of a. *p < 0.001 vs. all. Control (n = 15 cells); mock (n = 20 cells); XIP-NLS 40 (n = 15 cells); XIP-NLS 400 (n = 15 cells). c Superimposed traces of INCX currents recorded by patch-clamp in NGF-differentiated PC12 cells previously transfected with mock or XIP-NLS. d Quantification of c. mock (n = 20 cells); XIP-NLS (n = 20 cells)

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