Fig. 8: Effect of nuclear NCX1 inhibition on nuclear-resident PTEN/Akt pathway and on βIII-tubulin and MAP-2 immunostaining in mature cortical neurons. | Cell Death Discovery

Fig. 8: Effect of nuclear NCX1 inhibition on nuclear-resident PTEN/Akt pathway and on βIII-tubulin and MAP-2 immunostaining in mature cortical neurons.

From: Na+/Ca2+ exchanger 1 on nuclear envelope controls PTEN/Akt pathway via nucleoplasmic Ca2+ regulation during neuronal differentiation

Fig. 8

a Representative Western blots and quantifications of PTEN expression and Akt phosphorylation in nuclear preparations from mature cortical neurons (7DIV) transfected at 5 DIV with mock or XIP-NLS. All the experiments were repeated at least three times on different preparations. *p < 0.001 vs. mock. b Top: βIII-tubulin and MAP-2 staining in cortical neurons at 7 DIV under control conditions and in neurons transfected with XIP-NLS (bar = 20 μm; nuclei Hoechst blue). Bottom: Quantification of MAP-2 and βIII-tubulin fluorescence and % of their colocalization in cortical neurons at 7 DIV

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