Fig. 4: Sotetsuflavone inhibited VEGF and angiostatin expression. | Cell Death Discovery

Fig. 4: Sotetsuflavone inhibited VEGF and angiostatin expression.

From: Sotetsuflavone suppresses invasion and metastasis in non-small-cell lung cancer A549 cells by reversing EMT via the TNF-α/NF-κB and PI3K/AKT signaling pathway

Fig. 4

a Western blotting detection of VEGF protein expression. Using the same sample lysates as used for Fig. 2a, another gel (gel C) was run and probed with anti-VEGF. The GAPDH loading control is from gel A, and is the same as shown in Fig. 2a. b The expression of VEGF gene detected by RT-PCR. c Immunofluorescence assay detected the expression of angiostatin. Scale bar 50 μm. The data in the histogram represented the mean ± SD from three independent experiments (**P < 0.01, ***P < 0.001)

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