Fig. 7: Sotetsuflavone inhibited PI3K/AKT signaling pathway.

a Results from western blotting showing expression of PI3K and AKT protein. Using the same sample lysates as used for Fig. 2a, another gel (gel F) was run, the blot was cut horizontally, and probed with anti-PI3K and anti-AKT. The GAPDH loading control is from gel A, and is the same as shown in Fig. 2a. b Results from RT-PCR shows the expression of PI3K and AKT mRNA. The data in the histogram represented the mean ± SD from three independent experiments (**P < 0.01, ***P < 0.001)