Fig. 5: Cell-dependent regulation of TGF-β expression and secretion by 5-FU.

HuH-7, Hep G2, and AML-12 cells were treated with different concentrations of 5-FU as indicated (a), and 30 µg/mL 5-FU for the indicated time periods (b). TGF-β was detected by western blotting. TGF-β secretion was measured in HuH-7 and Hep G2 cells as described in Materials and Methods (c, d). Results are expressed as the means ± SD (n = 3)