Fig. 6: Responses of different cells to 5-FU, IFNα-2b, and combined treatment with respect to TGF-β levels and TGF-β-mediated signaling. | Cell Death Discovery

Fig. 6: Responses of different cells to 5-FU, IFNα-2b, and combined treatment with respect to TGF-β levels and TGF-β-mediated signaling.

From: Regulation of transforming growth factor is involved in the efficacy of combined 5-fluorouracil and interferon alpha-2b therapy of advanced hepatocellular carcinoma

Fig. 6

Hep G2, HuH-7, and AML-12 cells were individually treated with 5-FU, IFNα-2b, and a combination of 5-FU and IFNα-2b for 24 h. TGF-β levels in cell lysates (a) and culture supernatants (b) were detected. (c) Both Hep G2 and HuH-7 cells were treated with 5-FU for different periods as indicated. The levels of E-cadherin, p-SMAD2, and p15INK4b were detected by western blot. Actin was detected as an internal control. Results are expressed as the means ± SD (n = 3)

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