Fig. 6: Oxa12 decreases TNF-α gene expression and protein secretion levels.
From: Phenotypic screening identifies a new oxazolone inhibitor of necroptosis and neuroinflammation
a BV2 cells were incubated with zVAD-fmk (25 µM) in the presence or absence of Nec-1 (30 µM) or Oxa12 (30 µM) for 24 h. TNF-α mRNA levels were measured by qRT-PCR and secreted TNF-α by ELISA. Results are expressed as mean ± SEM from three independent experiments. *p < 0.001 vs. control; ǂp < 0.001 vs. zVAD-fmk. b BV2 cells were incubated with 100 ng/mL LPS, LPS plus zVAD-fmk (25 µM) in the presence or absence of Nec-1 (30 µM) or Oxa12 (30 µM) for 24 h. TNF-α and IL-1β mRNA levels were measured and results are expressed as mean ± SEM from three independent experiments. *p < 0.001 vs. control; Ɨp < 0.001 vs. LPS; ¶p < 0.001 vs. LPS/zVAD-fmk
