Fig. 2: TGF-β1 increases Kindlin-2 expression in HSCs via p38 and ERK MAPK.
From: Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis
a Representative western blot analysis of Kindlin-2 from LX-2 cells treated with TGF-β1 (10 ng/ml) for the indicated time period. The right panel shows quantitative analyses of Kindlin-2 after normalization with tubulin. b Representative western blot analysis of Kindlin-2 from LX-2 cells treated with TGF-β1 for 24 h for indicated concentrations. c Quantitative PCR analyses of Kindlin-2 mRNA from LX-2 cells treated with indicated concentrations of TGF-β1 for 24 h (left panel) or 10 ng/ml of TGF-β1 for indicated time intervals (right panel). Western blot (d) and immunofluorescence analysis (e) of Kindlin-2 from LX-2 cells were treated with or without SP600125 (10 μM), U0126 (1 μM), or SB203580 (1 μM), following TGF-β1 treatment for 24 h. The data are representative of three independent experiments. Scale bars = 200 μm. *p < 0.05. **p < 0.01
