Fig. 5: Kindlin-2 promotes Smad2/3 phosphorylation.
From: Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis
a LX-2 cells transfected with control siRNA or Kindlin-2 siRNA and further treated with 10 ng/ml TGF-β1 for the indicated time period. P-Smad2/3 and total Smad2/3 levels were detected by western blot. b LX-2 cells transfected with control siRNA or Kindlin-2 siRNA and further treated with 10 ng/ml TGF-β1 for 30 min. The expressions of P-Smad2/3 and total Smad2/3 were determined by immunofluorescence assays. c LX-2 cells were transfected with control vector or Kindlin-2 vector, followed by TGF-β1 (10 ng/ml) treatment for the indicated time period. P-Smad2/3 and total Smad2/3 levels were detected by western blot. d LX-2 cells were transfected with control vector or Kindlin-2 vector, followed by TGF-β1 (10 ng/ml) treatment for 30 min. The expressions of P-Smad2/3 and total Smad2/3 were determined by immunofluorescence assays. The data are representative of three independent experiments. Scale bars = 200 μm
