Fig. 2: BTK increases the expression of p21, PUMA and MDM2 in the absence of p53. | Cell Death Discovery

Fig. 2: BTK increases the expression of p21, PUMA and MDM2 in the absence of p53.

From: BTK modulates p73 activity to induce apoptosis independently of p53

Fig. 2

a Representative Western blot of lysates of HCT116p53−/− transfected with BTK (+) or an empty vector (−) and treated or not with 1.5 µM doxorubicin for 48, 24 h after transfection, showing levels of p21, PUMA, MDM2 and BTK. B-actin is used as a loading control. b Relative luciferase activity of p21, PUMA and MDM2 reporters in HCT116p53−/− transfected 24 h before with an empty vector (pCDNA3) or a BTK expression vector, in the absence or presence of 1.5 µM doxorubicin for 24 h. Graphs show average and standard deviations of three independent experiments. c mRNA levels, as measured by qRT-PCR, in the same cells. Graphs show average and standard deviations of three independent experiments

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