Fig. 3: Inhibition of p53 blocks the p53-independent pro-apoptotic activity of BTK.
From: BTK modulates p73 activity to induce apoptosis independently of p53
a Representative Western blot of lysates of HCT116p53−/− and H1299 transfected with control or p73 siRNA for 3 days, showing p73 protein levels. b Percentage of dead cells, as measured by Annexin V staining, in HCT116p53−/− transfected with an empty vector (pCDNA3), a BTK expression vector and/or p73 siRNA, treated or not with doxorubicin, as described above. Graphs show average and standard deviations of three independent experiments. c mRNA expression of p21 and PUMA, as measured with qRT-PCR, in HCT116p53−/− and H1299 transfected with an empty vector (pCDNA3), a BTK expression vector, p73 siRNA and/or a control siRNA. Graphs show average and standard deviations of three independent experiments performed in triplicate. Statistical significance is shown in comparison to the first column of each graph
