Fig. 4: A positive feedback loop between BTK and p73.
From: BTK modulates p73 activity to induce apoptosis independently of p53
a mRNA expression of p73, as measured with qRT-PCR, in HCT116p53−/− transfected with an empty vector (pCDNA3) or a BTK expression vector, in the absence or presence of 1.5 µM doxorubicin for 24 h. Graphs show average and standard deviations of three independent experiments performed in triplicate. b Representative Western blot of lysates of HCT116p53−/− transfected with a empty vector (−) or a BTK expression vector (+) 24 h before being treated or not with 1.5 µM doxorubicin for 24 h, showing p21, PUMA, p73 and BTK protein levels. c mRNA expression of p73, as measured with qRT-PCR, in HCT116p53−/− transfected with a control shRNA (shLuci) or an shRNA against BTK expression vector, in the absence or presence of 1.5 µM doxorubicin for 24 h. Graphs show average and standard deviations of three independent experiments performed in triplicate
