Fig. 5: Function of NDRG2 in maintaining photoreceptor cell viability and counteracting oxidative stress.
From: NDRG2 suppression as a molecular hallmark of photoreceptor-specific cell death in the mouse retina
a, b qRT-PCR analysis of the mRNA expression level (a) and western blot analysis of the protein expression level (b) of NDRG2 in the 661 W cell line. 661 W cells were transduced with either green fluorescent protein (GFP)-based lentiviral vector for NDRG2 overexpression (the NDRG2 group) or the vacant vector (the GFP group). c, d qRT-PCR analysis of the mRNA expression level (c) and western blot analysis of the protein expression level (d) of NDRG2 in the 661 W cell line. 661 W cells were transfected with either a short heparin RNA (shRNA) for NDRG2 knockdown (the shNDRG2 group) or the scrambled shRNA (the scramble group). e-g qRT-PCR analysis of the mRNA expression levels of NDRG1, 3 and 4 in the 661 W cell line upon NDRG2 manipulations. h-j MTT analysis of cell viability of the 661 W cell line upon NDRG2 manipulations. k, l qRT-PCR analysis of the mRNA expression levels of the antioxidant master transcription factor Nuclear factor E2-related factor 2 (Nrf2), the regulated antioxidant genes Heme oxygenase-1 (Ho-1) and NAD(P)H:quinone oxidoreductase-1 (Nqo-1) in the 661 W cell line upon NDRG2 manipulations. N = 3 per group. Data represents mean ± SD. *P < 0.05
