Fig. 6: Enhanced involution in autophagy gene deficient mice. | Cell Death Discovery

Fig. 6: Enhanced involution in autophagy gene deficient mice.

From: Autophagy and unfolded protein response (UPR) regulate mammary gland involution by restraining apoptosis-driven irreversible changes

Fig. 6

An involution time course was created using wild type (Atg7+/+) and Atg7 heterozygous (Atg7+/−) mice. Samples were collected as in Figs. 2–4 and as described in the Materials and methods. a, b H&E staining (top panels), p62 IHC (middle panels), and TUNEL IHC (bottom panels) of mammary glands of Atg7+/+ and Atg7+/− mice after 24 h, 48 h, and 72 h of involution. c, d Quantification of epithelial/adipose tissue area of the H&E stained slides as in Fig. 2, and as described in the Materials and methods, and in late involution (96 h, 7 d) by using ImageJ analysis, as described in the Materials and methods. Average ± SD are shown, n = 3–5. Student’s t test, P = 0.026 (96 h), P = 0.001 (7 d)

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