Fig. 1
From: Survival of midbrain dopamine neurons depends on the Bcl2 factor Mcl1
a. Mcl1 is abundantly expressed in mouse midbrain dopaminergic neurons. 2-day old Pitx3/GFP mice were killed, brains were isolated, and dissociated with papain followed by FACsorting. GFP-positive cells were collected and RNA was purified before RT-qPCR analysis with primers designed to specifically detect indicated transcripts. b Vulnerability of MN9D and N2A cell lines to a small molecule screen of apoptosis-inducing compounds. MN9D and N2A cells were incubated for 16 h at concentrations indicated. Cells were then treated with 0.1% Triton X-100 for 30 min, followed by 30 min incubation in DEVD-AMC (10 µM), a fluorogenic caspase 3 substrate. Fluorescence intensity was measured using a BioTek Synergy H1 plate reader. c Relative difference in fold change of cleaved caspase 3 activity of MN9D vs N2A cells. (n = 3, mean ± SD. Unpaired Student’s t-test, p-value: **P < 0.01)
