Fig. 3: UMI-77-dependent cell death requires Bax activation.
From: Survival of midbrain dopamine neurons depends on the Bcl2 factor Mcl1
a MN9D cells were treated with UMI-77 for 4 h followed by PFA fixation and staining with indicated antibodies. Scale bar represents 50 µm. Blow-up shows cell triple positive for DAPI, cleaved caspase 3 and Bax6A7. Scale bar represents 5 µm. b Bar graphs indicate percentage of cleaved caspase 3 positive cells relative to total cells, percentage of Bax6A7 positive cells relative to total cells (c) and percentage of cleaved caspase 3 cells which are also positive for Bax6A7 (d). e Western blot of cells pre-incubated with BIP-V5 before UMI-77 application for 4 h. Western was detected with various antibodies as indicated. Significance was determined with a Student’s t-test, p value: *p < 0.05, **p < 0.01 (n = 3)
