Fig. 5: Mcl1 is required for dopaminergic survival ex vivo.
From: Survival of midbrain dopamine neurons depends on the Bcl2 factor Mcl1
a Brains from adult Pitx3/GFP heterozygous mice were sliced, hemispheres were separated and either incubated overnight with vehicle or UMI-77 before fixation with PFA and staining for GFP, cleaved caspase 3 and total nuclei. Hemispheres were matched so that every treated hemisphere had its complementary hemisphere as control. After staining results were visualized and quantified. Data as indicated was obtained from the area indicated in the red square. Scale bar represents 100 µm. b Quantification of total amount of DAPI positive cells, GFP-positive cells, and cleaved caspase 3 and GFP-positive cells. **p < 0.01 (n = 7) as determined with a Student’s t-test
