Fig. 2: Gene and protein expression in amniotic fluid-derived stem cells (AFSCs) following cardiac differentiation. | Cell Death Discovery

Fig. 2: Gene and protein expression in amniotic fluid-derived stem cells (AFSCs) following cardiac differentiation.

From: The biochemical and electrophysiological profiles of amniotic fluid-derived stem cells following Wnt signaling modulation cardiac differentiation

Fig. 2

a Schematic representation of the cardiac differentiation protocol involving the modulation of Wnt signaling. b Gene expression of cardiac troponin T (cTnT) and myosin light chain 2 v (MLC2v) in those differentiating AFSCs since day 10 of cardiac differentiation was confirmed by reverse transcription-polymerase chain reaction (RT-PCR). P, positive control (human embryonic stem cell-derived cardiomyocytes, hESC-CMs); Un, undifferentiated AFSCs; 0, differentiation day 0; 5, differentiation day 5; 10, differentiation day 10; 14, differentiation day 14. c Relative gene expression of cTnT was measured by quantitative RT-PCR (qRT-PCR). Compared to undifferentiated AFSCs, cTnT gene expression was significantly increased since day 5 of cardiac differentiation. d Flow cytometry analysis of cell surface markers staining determined the percentage of cTnT-positive, MLC2a-positive, and MLC2v-positive cells after 14-day cardiac differentiation. Isotype controls are in blue and the surface markers are in red. Expression of each surface marker or isotype control was analyzed on 100,000 cells. The percentages of hESC-CMs expressing cTnT, MLC2a, and MLC2v were 93.2, 41, and 74.2%, respectively. The percentages of differentiated AFSCs expressing cTnT, MLC2a, and MLC2v were 60.2, 0.1, and 27.5%, respectively. Compared to hESC-CMs, the differentiated AFSCs did not show a typical bimodal distribution of cardiac differentiation

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