Fig. 3: Immunofluorescence staining in amniotic fluid derived stem cells (AFSCs) and human embryonic stem cells (hESCs) following cardiac differentiation.

Immunostaining for a cardiac troponin T (cTnT), b myosin light chain (MLC)2v, and c MLC2a detection in differentiated AFSCs and hESC-derived cardiomyocytes (hESC-CMs). The differentiated AFSCs expressed cTnT and MLC2v but not MLC2a. This finding was compatible with the gene expression results. These results suggested that AFSCs after 14 days of cardiac differentiation were cardiac ventricular-like cells. Scale bars represent 200 µm