Fig. 5: Effects of 2-deoxyglucose (2-DG), metformin (MET) and 2-DG+MET on the apoptosis of WT9-7 cells, as analyzed using annexin V/propidium iodide (PI) staining by flow cytometry (FCM) and a caspase-3 activity assay kit. | Cell Death Discovery

Fig. 5: Effects of 2-deoxyglucose (2-DG), metformin (MET) and 2-DG+MET on the apoptosis of WT9-7 cells, as analyzed using annexin V/propidium iodide (PI) staining by flow cytometry (FCM) and a caspase-3 activity assay kit.

From: Low-dose 2-deoxyglucose and metformin synergically inhibit proliferation of human polycystic kidney cells by modulating glucose metabolism

Fig. 5

a Results from analyzing the apoptosis rate (D1: necrosis; D2: late apoptosis; D3: living; D4: early apoptosis) by FCM in WT9-7 cells treated with 5 mM 2-DG, 2.5 mM MET and their combination for 48 h. b Caspase-3 activity in WT9-7 cells treated with 5 mM 2-DG, 2.5 mM MET and their combination for 12, 24, 36, and 48 h; ***p < 0.001 vs Control

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