Fig. 6: Effects of 2-deoxyglucose (2-DG) and metformin (MET) on the metabolism of WT9-7 cells.

a, b The real-time mitochondrial bioenergetic profiles and glycolytic profiles were generated using the Seahorse XF96 Analyzer. c, d Quantitative analysis of the total baseline and stressed bioenergetic profiles for the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of the four groups of WT9-7 cells; *p < 0.05,**p < 0.01, ***p < 0.001 vs Control, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs 2-DG, ^▲p < 0.05, ^▲▲p < 0.01, ^▲▲▲p < 0.001 vs MET. e Cell energy phenotypes were generated using the Seahorse XF96 Analyzer. f WT9-7 cells were treated with 5 mM 2-DG and 2.5 mM MET for 48 h and their glucose uptake was measured; *p < 0.05, **p < 0.01, ***p < 0.001 vs Control, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs 2-DG, ^▲p < 0.05, ^▲▲p < 0.01, ^▲▲▲p < 0.001 vs MET. g WT9-7 cells were treated with 5 mM 2-DG and 2.5 mM MET for 48 h and their lactate production was measured; *p < 0.05, **p < 0.01, ***p < 0.001 vs Control, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs 2-DG, ^▲p < 0.05, ^▲▲p < 0.01, ^▲▲▲p < 0.001 vs MET. h WT9-7 cells were treated with 5 mM 2-DG and 2.5 mM MET for 48 h and their ATP production was measured; *p < 0.05,**p < 0.01, ***p < 0.001 vs Control, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs 2-DG, ^▲p < 0.05, ^▲▲p < 0.01, ^▲▲▲p < 0.001 vs MET