Fig. 2: Identification of new TRIAD3 interacting proteins by mass spectrometry. | Cell Death Discovery

Fig. 2: Identification of new TRIAD3 interacting proteins by mass spectrometry.

From: TRIAD3/RNF216 E3 ligase specifically synthesises K63-linked ubiquitin chains and is inactivated by mutations associated with Gordon Holmes syndrome

Fig. 2

a List of potential new TRIAD3 interactors from mouse cortex lysate. HA-TRIAD3B was affinity purified from HEK293T lysate and incubated with mouse cortex lysate. Interacting proteins were identified by mass spectrometry. Only proteins that were identified in 3 out of 3 experiments with more than 10-fold enrichment over controls were considered. b STRING analysis shows network between identified proteins. The type of evidence is illustrated by edge colour: blue—from curated database, magenta—experimentally determined, green—textmining. Disconnected nodes were removed. Analysis was performed with medium confidence setting (0.400). c Ingenuity Pathway Analysis (IPA) predicts canonical pathways for the putative interactors shown in a. The 10 most significantly enriched pathways are shown, pathways specific to individual cancer cell types and cytotoxic T-lymphocytes were removed

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