Fig. 5: IGF-1 regulates Notch signaling and morphology in astrocytes.
From: Notch signaling in astrocytes mediates their morphological response to an inflammatory challenge
a IGF-1-induced Hes-5 overexpression involves IGF-1R. b. c IGF-1 increases Hes-5 transcription (b) and NICD production (c) through MAPK pathway. **, ***significant differences (p < 0.01 and p < 0.001) versus control cells. &&, &&&significant differences (p < 0.01 and p < 0.001) versus IGF-1-treated cells. d To determine whether exogenous addition of IGF-1 is able to modify astrocyte morphology, primary cultures were treated with 100 nM IGF-1 alone or in the presence of DAPT. Graph represents the percentage of cell type in each condition. Statistical significance was determined using one-way ANOVA and Bonferroni post hoc test for each phenotype separately, **, ***(p < 0.01 and p < 0.001) versus their respective control; &&&(p < 0.001) versus IGF-1-treated astrocytes, n = 4. Note that the effect of IGF-1 on astrocyte morphology is similar to NICD expression and opposite to LPS treatment
