Fig. 6: Chronic supplementation of FAC and CSE to culture media over 2 weeks leads to both increased number and area of autofluorescent particles in daily POS-fed hiPSC-RPE cultures. | Cell Death Discovery

Fig. 6: Chronic supplementation of FAC and CSE to culture media over 2 weeks leads to both increased number and area of autofluorescent particles in daily POS-fed hiPSC-RPE cultures.

From: Environmental stress impairs photoreceptor outer segment (POS) phagocytosis and degradation and induces autofluorescent material accumulation in hiPSC-RPE cells

Fig. 6

a Representative confocal images from three distinct trials showing increased accumulation of autofluorescent particles (red; spectral wavelength ex: 546 nm, em: 560–615 nm) in FAC + CSE-treated (200 µg/ml + 0.5%) and daily POS-fed (20 POS/RPE/cell) hiPSC-RPE cultures compared to untreated but daily POS-fed hiPSC-RPE cultures. Of note Hoescht-stained RPE cell nuclei are seen in blue. b–c Quantitative analysis of autofluorescence in untreated and POS-fed hiPSC-RPE cultures versus POS-fed FAC + CSE-treated hiPSC-RPE cultures in three distinct trials showed both increased number (c) and area (d) of autofluorescent particles in POS-fed FAC + CSE-treated hiPSC-RPE cultures in each individual trial. Data are presented as mean ± SEM. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05. n = 3 independent trials

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