Fig. 1: Krm1 homodimerizes through its extracellular domain.
From: Kremen1-induced cell death is regulated by homo- and heterodimerization
a Lysates from HEK293T cells transfected with Flag-Krm1 and HA-tagged full-length or truncation mutants of Krm1 lacking either the extracellular domain (ΔECD), the intracellular domain (ΔICD), or the transmembrane and intracellular domains (secECD) and subjected to anti-Flag immunoprecipitation followed by western blot using an anti-HA. An anti-panCadherin was used as control. Straight western blots on lysates are shown on the bottom panels. b Surface (green) and total (red) HA immunostaining of HEK293T cells transfected with HA-Krm1secECD together with either Green Fluorescent Protein (GFP, top lane) or full-length Krm1 (bottom lane). Scale bar: 10 µm. c Western blots and immunoprecipitation following extracellular proteins crosslinking with BS3 of HEK293T cells transfected with HA-Krm1 and Flag-Krm1
