Fig. 4: Krm2 inhibits Krm1 dimerization and apoptotic signaling.
From: Kremen1-induced cell death is regulated by homo- and heterodimerization
a Lysates from HEK293T cells transfected with Flag-Krm1 and HA-Krm1 together with either GFP, LRP6, Krm2, or EpCAM and subjected to anti-Flag immunoprecipitation followed by anti-HA western blot. An anti-panCadherin was used as control. Straight western blots on lysates are shown on the bottom panels. b Cleaved Caspase-3 (green) and HA (red) immunostaining of HEK293T cells transfected with HA-Krm1 and either GFP or Krm2 constructs. The histogram represents the proportion of Caspase-3+ cells among HA+ cells (average ± sd) following immunostaining. *p < 0.001 using Student’s t-test. Scale bar: 10 µm
