Fig. 4: Mefloquine-induced ROS production is dependent on iron.
From: Lysosomotropic challenge of mast cells causes intra-granular reactive oxygen species production
a Mast cells were preincubated ± deferoxamine (DFO; 100 μM; for 24 h), followed by incubation ± mefloquine (20 μM). Cellular levels of ROS were assessed by flow cytometry after 30 min. Cells treated with DFO alone were included as controls. The shown data represent means ± SEM (n = 3). b Total iron, copper, manganese and zinc levels in WT and serglycin−/− (SG−/−) mast cells. Metal content was measured by inductively coupled plasma-mass spectrometry. The shown data represent means ± SEM (n = 3−5). c Relative LFQ intensities for ferritin in PBS- and mefloquine-treated WT and SG−/− mast cells. WT and SG−/− mast cells were incubated ± mefloquine (20 μM) and after 1 h cells were harvested and subjected to MS-based proteomics analysis. The data shown represent means ± SEM (n = 4). d Mast cells were preincubated ± apocynin (APO; 100 μM) for 24 h, followed by incubation ± mefloquine (20 μM). Cellular levels of ROS were assessed by flow cytometry after 30 min of incubation. Data are presented as means ± SEM (n = 3)
