Fig. 4: Curcumin induces autophagy pathway in Ana2014 cells. | Cell Death Discovery

Fig. 4: Curcumin induces autophagy pathway in Ana2014 cells.

From: Curcumin induced oxidative stress causes autophagy and apoptosis in bovine leucocytes transformed by Theileria annulata

Fig. 4

a Confocal microscopy of Ana2014 cells treated with curcumin (20 µM) showing the accumulation of many acridine orange (AO) positive acidic vesicles, b Western blot analysis of Ana2014 cells treated with various concentrations (5, 10, and 20 µM) of curcumin showing the conversion of LC3A to LC3B, c Fold change in expression of LC3B/A after normalization of western blot data. N = 3. Data are presented as mean ± SD. * represents p < 0.05, ** represents p < 0.01, and *** represents p < 0.001, compared with the untreated group, d Ana2014 cells transiently transfected with mCherry-hLC3B-pcDNA3.1 were treated with 20 µM curcumin. mCherry-hLC3B-pcDNA3.1 puncta were observed by confocal microscope, e significant increase in the number of puncta per cell upon curcumin treatment

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