Fig. 3: PAP activity and accumulation of PA exhibited by PfPAP2 in malaria parasite. | Cell Death Discovery

Fig. 3: PAP activity and accumulation of PA exhibited by PfPAP2 in malaria parasite.

From: Phosphatidic acid homeostasis regulated by a type-2 phosphatidic acid phosphatase represents a novel druggable target in malaria intervention

Fig. 3: PAP activity and accumulation of PA exhibited by PfPAP2 in malaria parasite.

a Lysate of mature blood stages of malaria parasites demonstrated PA dependent phosphatase activity. Lysate of mature blood stages of parasite release Pi in the presence of PA which is inhibited by propranolol (125uM) indicating activity of PfPAP2. **P < 0.01, (n = 3). b Lysate of mature blood stages of malaria parasites accumulates PA in the presence of propranolol (125 μM). **P < 0.01, (n = 2). c (i) PA accumulation in parasite lysate in the presence of propranolol (125 μM) was monitored by LC-MS which demonstrated higher intensity peak of 647.29 m/z that corresponds to PA. (ii) Quantification of LC-MS peak intensity establishes accumulation of PA in response to propranolol

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