Fig. 4: FKBP51s regulates PD-L1 expression and spheroid formation in TM-GBM and SVZ-GBM cells. | Cell Death Discovery

Fig. 4: FKBP51s regulates PD-L1 expression and spheroid formation in TM-GBM and SVZ-GBM cells.

From: The splicing FK506-binding protein-51 isoform plays a role in glioblastoma resistance through programmed cell death ligand-1 expression regulation

Fig. 4: FKBP51s regulates PD-L1 expression and spheroid formation in TM-GBM and SVZ-GBM cells.

a Immunoblot of PD-L1 and FKBP51s levels in cells silenced for FKBP51s. Canonical FKBP51 confirmed the specificity of the silencing. b Flow cytometry analysis of PD-L1 expression in cells, silenced or not silenced for FKBP51s. Graph columns represent MFI using non-silenced cells as the reference sample (N = 3). c Spheroid assay with cells from TM-GBM and SVZ-GBM cells, silenced or not silenced for FKBP51s. Representative images of formed spheroids are shown. Graph columns represent the relative spheroid numbers, after a 4-day culture, using NSRNA-treated cells as the reference sample (N = 3). d Spheroid assay with TM-GBM cells and SVZ-GBM cells transfected with EV, PD-L1-GFP+ NSRNA, and PD-L1-GFP + FKBP51s siRNA. FKBP51s depletion reduced the spheroid formation stimulated by exogenous PD-L1 (scale bar represents 250μm) (N = 3). e Fluorescent microscopy visualization of ectopic PD-L1-GFP in the spheroid assay (scale bar represents 50 μm). FKBP51s depletion reduced spheroid numbers and the extent of fluorescence. f Western blot assay of FKBP51s levels in FKBP51s-silenced spheroids. Each experiment was performed at least three times and in triplicate

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