Fig. 4: TSC2 plays a key role in the down regulation of MTORC1 in response to glucose energetic stress. | Cell Death Discovery

Fig. 4: TSC2 plays a key role in the down regulation of MTORC1 in response to glucose energetic stress.

From: Essential role of glucokinase in the protection of pancreatic β cells to the glucose energetic status

Fig. 4: TSC2 plays a key role in the down regulation of MTORC1 in response to glucose energetic stress.The alternative text for this image may have been generated using AI.

a Western blot analysis (n = 3) of MEF TSC2+/+ and TSC2 −/− cells challenged to different stimuli for 3 h with its corresponding quantification and statistical analysis. The mTORC1 activity (%) represents the percentage of change in P-p70 (Thr 389)/p/70 ratio. *P < 0.05 and **P < 0.01 indicates the comparison between control with either metformin, 2DG or glucose deprivation in MEF TSC2+/+ cells. b Western blot analysis (n = 3) of MIN6 Scr and TSC2 shRNA cells stimulated with either 2DG or glucose deprivation for 3 h. *P < 0.05, t-test (two-tailed, unpaired), comparing control with either 2DG or glucose deprivation in MIN6 scrambled (Scr) cells. c Western blot analysis (n = 3) of MIN6 Scr and TSC2 shRNA cells stimulated with metformin for 3 h. *P < 0.05, t-test (two-tailed, unpaired), is the value corresponding to the comparison between control and metformin treatment in MIN6 Scr cells

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