Figure 3: Immunofluorescence staining of thick (30 µm) sections viewed on a confocal microscope, comparing constrained (right) and unconstrained (left) disc explants.
From: Physical disruption of intervertebral disc promotes cell clustering and a degenerative phenotype
Red-staining for antibodies refer to: MMP-1 (a, b), integrin α5β1 (c, d), and denatured collagen type II (e). Cell nuclei are stained blue with Dapi. In f, ‘Volocity’ image analysis software was used to measure the areas of denatured collagen, which colour coded and analysed positive stained area in 30-µm-thick section as clear blue, red, green, and blue colour. Scale bar = 50 µm.
