Figure 4: Visual comparisons between constrained and unconstrained disc tissue.
From: Physical disruption of intervertebral disc promotes cell clustering and a degenerative phenotype

a Surgically removed intervertebral disc tissue. b Perspex ring used for constraining the 5 mm3 disc tissue blocks. c Disc tissue in the perspex ring as ‘constrained tissue’ that was refrained from swelling for 36 h. d ‘Unconstrained’ free swelling disc after 36 h.