Fig. 5: Neurogenesis in ischemic cortex is activated and anxiety-like behavior is relieved by administering As-IV. | Cell Death Discovery

Fig. 5: Neurogenesis in ischemic cortex is activated and anxiety-like behavior is relieved by administering As-IV.

From: Antagonistic effects of IL-17 and Astragaloside IV on cortical neurogenesis and cognitive behavior after stroke in adult mice through Akt/GSK-3β pathway

Fig. 5: Neurogenesis in ischemic cortex is activated and anxiety-like behavior is relieved by administering As-IV.Fig. 5: Neurogenesis in ischemic cortex is activated and anxiety-like behavior is relieved by administering As-IV.

a Experimental scheme for assessing NSCs’ stemness and neurogenesis in ischemic cortex. b, c Double-immunostaining of Nestin (green)/GFAP (red) at 3 dpi and BrdU (green)/DCX (red) at 7 dpi after stroke. d Notice the increase of double positive cells numbers of Nestin+-GFAP+ in infarcted area per square millimeter. Data represent mean ± SEM, n = 3; *P < 0.05. e Notice increased double positive cells numbers of BrdU+-DCX+ and BrdU+-DCX+/BrdU+ cells percentage in As-IV treated mice. Data represent mean ± SEM, n = 3; *P < 0.05, **P < 0.01. f Open field assay of sham, control and As-IV treated mice. g Notice the decrease of time and distance in center area in control group compared with the sham group. The time and distance in center area was increased in As-IV treated mice, compared with the control group. Data represent mean ± SEM, n = 7; *P < 0.05. h Elevated plus maze test of sham, control and As-IV treated mice. i Notice the decrease of time and distance in the open field and open arm in control group compared with the sham group. The time and distance in the open field and open arm was increased in As-IV treated mice, compared with the control group. Data represent mean ± SEM, n = 7; *P < 0.05, ***P < 0.001. As: Astragaloside IV; Con: control; GFAP: glial fibrillary acidic protein; DCX: doublecortin.

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