Fig. 8: Neurospheres’ diameter and NSCs’ proliferation ability is promoted by As-IV but inhibited by IL-17A in vitro. | Cell Death Discovery

Fig. 8: Neurospheres’ diameter and NSCs’ proliferation ability is promoted by As-IV but inhibited by IL-17A in vitro.

From: Antagonistic effects of IL-17 and Astragaloside IV on cortical neurogenesis and cognitive behavior after stroke in adult mice through Akt/GSK-3β pathway

Fig. 8: Neurospheres’ diameter and NSCs’ proliferation ability is promoted by As-IV but inhibited by IL-17A in vitro.

a Proliferation ability of viable NSCs in culture by treating PBS, As-IV and IL-17A respectively was analyzed. Notice the increase of NSCs’ diameter and proliferation ability in As-IV treated culture and decrease in IL-17A treated culture. Data represent mean ± SEM, results are representative of three independent experiments, *P < 0.05, **P < 0.01. b Western blotting and quantitative data for p-Akt, p-GSK-3β, GSK-3β, IL-17, Wnt2, and β-catenin in different culture treated PBS, IL-17A, As-IV and IL-17A + As-IV, respectively. c Notice the increased expression of p-Akt, p-GSK-3β, Wnt2, and β-catenin by treating As-IV, with IL-17 expression decreased. Notice the decreased expression of p-Akt, p-GSK-3β, Wnt2, and β-catenin by treating As-IV, with IL-17 expression increased. Significant increase of p-Akt, p-GSK-3β, Wnt2, and β-catenin protein expression was observed in IL-17A treated culture, with decreased IL-17. Data represent mean ± SEM, *P < 0.05, **P < 0.01. d Schematic drawing of the experimental design. As: Astragaloside IV; Con: control; IL: interleukin; PI3K: phosphatidylinositol-4,5-bisphosphate 3-kinase; GSK-3β: glycogen synthase kinase 3β; NPCs: neural precursor cells.

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