Fig. 3: Effects of Au-NPs on He-CAP induced intracellular ROS production. | Cell Death Discovery

Fig. 3: Effects of Au-NPs on He-CAP induced intracellular ROS production.

From: Small size gold nanoparticles enhance apoptosis-induced by cold atmospheric plasma via depletion of intracellular GSH and modification of oxidative stress

Fig. 3

The percentages of cells with elevated species of ROS were analyzed 3 h after He-CAP by flow cytometry. a DCFH-DA staining, b HE staining, c HPF staining. Results are represented as means ± S.E.M. of three independent experiments, **P < 0.001 and ***P < 0.0001 vs either treatment alone as determined by one-way ANOVA with Bonferroni’s multiple comparison test (S.E.M. is indicated by bars). d Intracellular GSH level, as measured using a G.S.H. kit and analyzed by flow cytometry. Results are represented as means ± S.E.M. of three independent experiments, *P < 0.05 compared with the control group and, **P < 0.001 compared to the He-CAP alone group evaluated by Student’s t test (S.E.M. is indicated by bars). e Assessment of DNA damage. The extent of H2AX phosphorylation was determined immediately after He-CAP treatment in the presence or absence of Au-NPs. β-actin was used to normalize the expression level of each sample. Blots were cropped, full-length blots are presented in Supplementary Fig. 2. f Assessment of apoptotic cell death in the presence of NAC, Annexin V-FITC/PI. Results are represented as means ± S.E.M. of three independent experiments, ***P < 0.0001 vs the combined treatment group as determined by two-way ANOVA with Bonferroni posttest (S.E.M. is indicated by bars). g Giemsa staining under a microscope at ×400 magnification. One representative photomicrograph is shown here, with the arrowhead showing apoptotic cells.

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