Fig. 2: MiR-185-5p aggravated the hyperoxia-induced necroptosis gene markers in lung epithelial cells.
From: Extracellular vesicle-cargo miR-185-5p reflects type II alveolar cell death after oxidative stress
a, b MLE15 cells were treated with room air or hyperoxia for 1 and 2 days. a RIPK1 gene expression and b RIPK3 gene expression level was measured using real-time PCR. c, d MLE15 cells were transfected with miRNA-185-5p mimics, and treated with hyperoxia for 1 day. c RIPK1 gene expression and d RIPK3 gene expression level was measured using real-time PCR. e MLE15 cells were treated with room air or hyperoxia for 1 day and RIPK1 and RIPK3 protein expression was measured by Western blot. f, g Protein levels were quantified and normalized to actin. The figures represent three independent experiments (n = 4 samples per group) with identical results. Mean ± SD; *p < 0.05, **p < 0.01.
